Isolation, Identification and Study of Antimicrobial Property of a Bioactive Compound in an Indian Medicinal Plant Acalypha indica (Indian-Nettle) |
| |
Authors: | R D Jebakumar Solomon Subramaniam Kallidass Jayaraj Vimalan |
| |
Institution: | (1) Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021, Tamilnadu, India |
| |
Abstract: | Summary Fresh, dried and powdered samples of leaf, stem and root of Acalypha indica were subjected to fractional distillation in a soxhlet apparatus using solvents such as hexane, chloroform, acetone and methanol.
The plant extracts and a synthetic antifungal compound, Clotrimazole (authentic standard) were subjected to TLC and HPLC analyses.
The Rf (relative front) value of Clotrimazole was 0.371. The plant’s leaf, root and stem extracts also gave distinct spots respectively
at Rf value of 0.371 ± 0.0009. In HPLC, the TLC-separated active compound and Clotrimazole resolved at 1.90 ± 0.2 min (retention
time). The amounts of active compound present in root, leaf and stem extracts were 538, 415 and 171 μg/g respectively. From the results of our study, we infer that the active compound isolated from Acalypha indica is more potent in controlling Candida albicans, Aspergillus niger and Escherichia coli. The Active compound present in the plant had more than 100% activity when compared to standard Clotrimazole. |
| |
Keywords: | Acalypha indica antimicrobial compound Aspergillus niger bioactive compound Candida albicans Clotrimazole Escherichia coli HPLC TLC |
本文献已被 SpringerLink 等数据库收录! |
|