Elasmobranch qPCR reference genes: a case study of hypoxia preconditioned epaulette sharks |
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| Authors: | Kalle T Rytkönen Gillian MC Renshaw Kevin J Ashton Grant Williams-Pritchard Erica H Leder Mikko Nikinmaa |
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| Affiliation: | (1) Division of Genetics and Physiology, Department of Biology, University of Turku, FI-20014 Turku, Finland;(2) Centre of Excellence in Evolutionary Genetics and Physiology, Department of Biology, University of Turku, Turku, Finland;(3) Hypoxia and Ischemia Research Unit, School of Physiotherapy and Exercise Science, Griffith University, Queensland, Australia;(4) Biomedical Science, Bond University, Queensland, Australia;(5) Heart Foundation Research Centre, Griffith University, Queensland, Australia |
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| Abstract: | Background Elasmobranch fishes are an ancient group of vertebrates which have high potential as model species for research into evolutionary physiology and genomics. However, no comparative studies have established suitable reference genes for quantitative PCR (qPCR) in elasmobranchs for any physiological conditions. Oxygen availability has been a major force shaping the physiological evolution of vertebrates, especially fishes. Here we examined the suitability of 9 reference candidates from various functional categories after a single hypoxic insult or after hypoxia preconditioning in epaulette shark (Hemiscyllium ocellatum). |
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