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rII cistrons of bacteriophage T4. DNA sequence around the intercistronic divide and positions of genetic landmarks
Authors:D Pribnow  D C Sigurdson  L Gold  B S Singer  C Napoli  J Brosius  T J Dull  H F Noller
Affiliation:Department of Molecular, Cellular and Developmental Biology University of Colorado, Boulder, Col. 80309, U.S.A.;Thimann Laboratories University of California, Santz Cruz, Calif. 95064, U.S.A.
Abstract:
An 873 base-pair DNA sequence from the rII region of bacteriophage T4 is presented. The sequence encodes 139 carboxyl-terminal amino acids of rIIA and the amino-terminal 146 amino acids of rIIB. Eleven base-pairs separate the rIIA stop codon (UAA) and the rIIB AUG.An extensive genetic map is superimposed on the DNA sequence, showing the deduced locations of many of the mutations (base-pair substitutions, frameshifts, deletions) found in previous rII genetic studies.
Keywords:SDS  sodium dodecyl sulfate
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