rII cistrons of bacteriophage T4. DNA sequence around the intercistronic divide and positions of genetic landmarks |
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Authors: | D Pribnow D C Sigurdson L Gold B S Singer C Napoli J Brosius T J Dull H F Noller |
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Affiliation: | Department of Molecular, Cellular and Developmental Biology University of Colorado, Boulder, Col. 80309, U.S.A.;Thimann Laboratories University of California, Santz Cruz, Calif. 95064, U.S.A. |
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Abstract: | ![]() An 873 base-pair DNA sequence from the rII region of bacteriophage T4 is presented. The sequence encodes 139 carboxyl-terminal amino acids of rIIA and the amino-terminal 146 amino acids of rIIB. Eleven base-pairs separate the rIIA stop codon (UAA) and the rIIB AUG.An extensive genetic map is superimposed on the DNA sequence, showing the deduced locations of many of the mutations (base-pair substitutions, frameshifts, deletions) found in previous rII genetic studies. |
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Keywords: | SDS sodium dodecyl sulfate |
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