Overexpression of AtALMT1 in the Arabidopsis thaliana ecotype Columbia results in enhanced Al-activated malate excretion and beneficial bacterium recruitment |
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Authors: | Yasufumi Kobayashi Venkatachalam Lakshmanan Yuriko Kobayashi Minatsu Asai Satoshi Iuchi Masatomo Kobayashi Harsh P Bais Hiroyuki Koyama |
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Institution: | 1.Laboratory of Plant Cell Technology; Faculty of Applied Biological Sciences; Gifu University; Gifu, Japan;2.Department of Plant and Soil Sciences; Delaware Biotechnology Institute; University of Delaware; Newark, DE USA;3.Experimental Plant Division; RIKEN-BRC; Tsukuba; Ibaraki, Japan |
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Abstract: | AtALMT1 (Arabidopsis thaliana ALuminum activated Malate Transporter 1) encodes an Arabidopsis thaliana malate transporter that has a pleiotropic role in Arabidopsis stress tolerance. Malate released through AtALMT1 protects the root tip from Al rhizotoxicity, and recruits beneficial rhizobacteria that induce plant immunity. To examine whether the overexpression of AtALMT1 can improve these traits, the gene, driven by the cauliflower mosaic virus 35S promoter, was introduced into the Arabidopsis ecotype Columbia. Overexpression of the gene enhanced both Al-activated malate excretion and the recruitment of beneficial bacteria Bacillus subtilis strain FB17. These findings suggest that overexpression of AtALMT1 can be used as an approach to enhance a plant's ability to release malate into the rhizosphere, which can enhance plant tolerance to some environmental stress factors. |
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Keywords: | Arabidopsis thaliana AtALMT1 Bacillus subtilisstrain FB17 Pseudomonas syringae pv tomato DC3000 aluminum malate exudation |
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