Ent-kaurene,occurrence and metabolism in Hordeum distichon |
| |
Authors: | George JP Murphy Dennis E Briggs |
| |
Institution: | Department of Biochemistry, University of Birmingham, P.O. Box 363, Birmingham B15 2TT U.K. |
| |
Abstract: | Barley grains contain hydrocarbons, including a material indistinguishable from ent-kaurene by GLC, and which after appropriate chemical conversions contain material behaving like ent-kauran-16,17-diol, ent-kaurene norketone and ent-17-nor-kaurane on TLC and GLC. The presence of ent-kaurene was confirmed by conversion to ent-kauran-16-ol and, following formation of acetate-3H], recrystallization to constant specific activity with unlabelled carrier. In the initial ca. 15 hr of germination, preceding the rise in endogenous gibberellins, the level of ent-kaurene falls. Exogenous ent-kaurene-14C] was not metabolized by intact barley grains. ent-Kauran-16,17-epoxide was formed non-enzymically by boiled extracts. Unboiled homogenates also formed ent-kauran-17-ol and ent-kauran-16,17-diol. The diol appeared to be formed from the epoxide, but the ent-kauran-17-ol was not. No recognized gibberellin precursors were detected. Nevertheless, endogenous ent-kaurene may be the stored biosynthetic precursor of gibberellins in germinating barley grains. |
| |
Keywords: | Gramineae barley estimation metabolism |
本文献已被 ScienceDirect 等数据库收录! |
|