Multiple forms of Vitis vinifera catechol oxidase

Grape catechol oxidase shows multiple forms upon ion exchange chromatography, acrylamide gel electrophoresis and gel filtration. Conversion of some bands into others, which occurs during isolation and storage, is enhanced by dilution and by treatment with urea or acid pH. Estimation of MWs suggested that the conversions might be due to dissociation of the enzyme into subunits, but attempts to induce reassociation were unsuccessful. The effects of urea and acid pH could be imitated by partial enzymic proteolysis of the enzyme. Analysis of the various bands observed in gel electrophoresis suggested that some of the enzyme forms have the same MWs but differ in charge distribution.