Effects of pyrethroid insecticides on gap junctional intercellular communications in Balb/c3T3 cells by dye-transfer assay |
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Authors: | Chise Tateno Seiichi Ito Mina Tanaka Akira Yoshitake |
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Institution: | (1) Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd, 1-98, 3-chome, Kasugade-naka, Konohana-ku, Osaka, Japan |
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Abstract: | The effects of fenvalerate, esfenvalerate, permethrin, cypermethrin, deltamethrin, p-chlorophenylisovaleric acid (CPIA, major metabolite of fenvalerate) and DDT, a liver tumor promoter, on gap junctional intercellular communication (GJIC) were examined in Balb/c3T3 cells by dye-transfer assay. Separate groups of Balb/c3T3 cells were exposed to the chemicals for 1 day. On the following day, GJIC was measured by counting the number of dye-transferring cells per injection of Lucifer Yellow under a fluorescent microscope. Fenvalerate, esfenvalerate, permethrin, cypermethrin, deltamethrin and DDT inhibited GJIC at noncytotoxic concentrations, while CPIA did not inhibit GJIC even, at a cytotoxic concentration. It is concluded that the examined pyrethroid insecticides, but not a metabolite, have inhibitory effects on GJIC in Balb/c3T3 cells.Abbreviations DDT
1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane
- DMSO
dimethyl sulfoxide |
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Keywords: | Balb/c3T3 cypermethrin DDT deltamethrin dye-transfer assay esfenvalerate fenvalerate permethrin pyrethroids intercellular communication |
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