| 用焦磷酸测序技术研究猪线粒体细胞色素B基因单倍型 |
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| 引用本文： | 蒋思文,熊远著,U.Gustafsson,E.Giuffra,L.Andersson.用焦磷酸测序技术研究猪线粒体细胞色素B基因单倍型[J].遗传,2001,23(2):119-122. |
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| 作者姓名： | 蒋思文 熊远著 U.Gustafsson E.Giuffra L.Andersson |
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| 作者单位： | 1.华中农业大学畜牧兽医学院，农业部猪遗传育种重点实验室，武汉 430070;
2.瑞典农业大学动物遗传育种系，瑞典
1.College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070；
2.Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala BMC, S-751 24 Uppsala, Sweden |
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| 基金项目： | 国家重大基础研究项目“973”（G2000016105）资助部分研究内容 |
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| 摘 要： | 选择43头大白猪，79头长白猪，66头皮特兰猪和60头清平猪作试验材料，采用焦磷酸测序技术分析猪线粒体细胞色素b(CytB)基因单倍型。研究结果显示CytB基因可分为4种单倍型E1，E2，A1和A2。清平猪仅存在于A1单倍型(100%)，大白猪和长白猪存在于E1(49.19%,79.25%)和A1(55.81%,20.25%)单倍型，皮特兰则存在于E1(57.58%)和A2(42.42%)单倍型。
Abstract：To detect porcine mitochondrial cytochrome b (CytB) gene haplotypes, Pyrosequencing, which is a novel DNA sequencing method, has been used to analyze SNPs selected Large White, Landrace, Pietrain and Qingping pigs. The pyrosequencing analysis of CytB gene displayed four distinct haplotypes E1, E2, A1 and A2 respectively. Qingping pigs are only present in haplotype A1, Large White and Landrace pigs are present in haplotype E1 and A1, and Pietrain pigs are present in haplotupe E1 and A2.
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| 关 键 词： | 线粒体DNA 细胞色素b基因 焦磷酸测序 Keywords 单倍型 mitochondrial DNA |
| 文章编号： | 0253-9772(2001)02-0119-04 |
| 修稿时间： | 2000年11月14 |
Porcine Mitochondrial
Cytochrome B Gene Haplotypes Detection by Pyrosequencing |
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| JIANG Si-Wen,XIONG Yuan-Zhu,U.Gustafsson,E.Giuffra,L.Andersson.Porcine Mitochondrial
Cytochrome B Gene Haplotypes Detection by Pyrosequencing[J].Hereditas,2001,23(2):119-122. |
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| Authors: | JIANG Si-Wen XIONG Yuan-Zhu UGustafsson EGiuffra LAndersson |
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| Institution: | JIANG Si-wen1,U.Gustafsson2,E. Giuffra2,L. Andersson2, XIONG Yuan-zhu1 |
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| Abstract: | To detect porcine mitochondrial cytochrome b (CytB) gene haplotypes, Pyrosequencing, which is a novel DNA sequencing method, has bee n used to analyze SNPs selected Large White, Landrace, Pietrain and Qingping pig s. The pyrosequencing analysis of CytB gene displayed four distinct haplotypes E 1, E 2, A 1 and A 2 respectively. Qingping pigs are only present i n haplotype A 1, Large White and Landrace pigs are present in haplotype E 1 and A 1, and Pietrain pigs are present in haplotupe E 1 and A 2. |
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| Keywords: | mitochondrial DNA cytochrome B gene haplotype pyrosequencing |
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