Tight binding ligand approach to oligosaccharide-grafted protein |
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| Authors: | Totani Kiichiro Matsuo Ichiro Ito Yukishige |
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| Affiliation: | CREST, JST, Kawaguchi 332-1102, Japan. |
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| Abstract: | ![]()
A novel type of artificial glycoprotein was developed, by using dihydrofolate reductase (DHFR) and methotrexate (MTX) as a protein-ligand pair. Various oligosaccharides linked to MTX were shown to bind tightly with DHFR and afforded oligosaccharide-grafted protein, which could be isolated easily by lectin beads. |
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