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Use of a cytoplasmically localised P-lacZ fusion to identify cell shapes by enhancer trapping inDrosophila
Authors:Hazel K Smith  Cahir J O'Kane
Institution:(1) Department of Biological Sciences, University of Warwick, CV4 7AL Coventry, UK
Abstract:Summary The N-terminal 125 amino acids of theDrosophila P element transposase are necessary and sufficient for the nuclear localisation of a hybridlacZ gene product in most cell types of theDrosophila embryo. A P-lacZ enhancer-trap element lacking these residues is of use in visualizing the shapes of P-lacZ-expressing cells.
Keywords:Cytoplasmic P-lacZ  Enhancer-trapping  Nuclear targeting
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