Plant regeneration from stem and petiole protoplasts of sweet potato (Ipomoea batatas) and its wild relative,I. lacunosa |
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Authors: | M M Belarmino T Abe T Sasahara |
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Institution: | (1) Laboratory of Plant Breeding, Faculty of Agriculture, Yamagata University, 997 Tsuruoka, Japan;(2) Present address: Philippine Root Crop Research & Training Center, ViSCA, 6521-A Leyte, Philippines |
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Abstract: | A protoplast-to-plant regeneration system has been established for sweet potato (Ipomoea batatas (L.) Lam.) and its wild relative, I. lacunosa L. Viable protoplasts, isolated from preplasmolyzed stems and petioles of in vitro-grown plants, were cultured on liquid MS (Murashige & Skoog 1962) medium that supported cell division and colony formation. Embryogenic calli of sweet potato were induced on agar-solidified MS medium supplemented with 3% (w/v) sucrose, 50 mg l-1 casamino acids, 0.2–0.5 mg l-1 2,4-d, 1.0 mg l-1 kinetin and 1.0 mg l-1 ABA. On average, 3 plants were regenerated from a single sweet potato callus subcultured on semi-solid MS medium containing 3% (w/v) sucrose, 800 mg l-1 glutamine, 2.0 mg l-1 BA or 1.0 mg l-1 kinetin and 1.0 mg l-1 GA3. Embryogenic calli of I. lacunosa L. were initiated on semi-solid MS medium containing 0.2–0.5 mg l-1 IAA and 1.0–2.0 mg l-1 BA. An average of 5 plants was regenerated from a single sweet potato callus subcultured on semi-solid MS medium containing 0.5 or 1.0 mg l-1 GA3.Abbreviations ABA
abscisic acid
- BA
benzyladenine
- 2,4-d
2,4-dichlorophenoxyacetic acid
- GA3
gibberellic acid
- IAA
indole acetic acid
- MES
2-(N-morpholino)-ethane sulfonic acid
- NAA
-naphthaleneacetic acid |
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Keywords: | embryogenic callus Ipomoea batatas I lacunosa plant regeneration protoplast |
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