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The high genetic homology of threeMacaca fascicularis and twoMacaca mulatta subspecies on the basis of their highly repeated DNA restriction patterns
Authors:S Crovella  M P Bigatti  G Ardito  M Del Pero  D Montagnon  L Lamberti
Institution:(1) Dipartimento di Biologia Animale, Università di Torino, Via Accademia Albertina 17, 10124 Torino, Italy;(2) Istituto dì Antropologia, Università di Firenze, Firenze, Italy;(3) Institut d’Embryologie, Universitè L. Pasteur, Strasbourg;(4) Dipartimento di Biologia Animale, Università di Torino, Torino, Italy
Abstract:We have studied highly repeated DNA sequences of three subspecies ofM. fascicularis (M.f. philippinensis M.f. mordax, M.f. fusca) and of two subspecies ofM. mulatta (M.m. lasiotus, M.m. mulatta). Restriction patterns were obtained after digestion with 9 restriction endonucleases and evidenced after southern blotting and hybridization with Bam HI satellite DNA fragments fromM. fascicularis subspecies. M. fascicularis andM. mulatta subspecies studied, present morphological differences but indistinguishable karyotypes: highly repeated DNA analysis, resulting in the same restriction patterns for all the restriction sites studied with highly repeated DNA probes characteristic of the threeM. fascicularis subspecies, gave arguments in favour of the high genetic homology ofM.f. philippinensis, M.f. mordax, M.f. fusca on one side, andM.m. lasiotus andM.m. mulatta on the other, which can be distinguished only on the basis of morphological criteria.
Keywords:Highly repeated DNA  restriction enzymes  southern blot            M  fascicularis  M  mulatta
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