| 小麦与禾草对称融合中杂种染色体组成与亲缘关系的初步探究 |
| |
| 引用本文： | 邢梅青,夏光敏,陈惠民.小麦与禾草对称融合中杂种染色体组成与亲缘关系的初步探究[J].植物研究,2001,21(1):74-78. |
| |
| 作者姓名： | 邢梅青 夏光敏 陈惠民 |
| |
| 作者单位： | 1. 山东省实验中学,
2. 山东大学, |
| |
| 摘 要： | 采用PEG法诱导普通小麦的原生质体与新麦草、无芒雀麦及韦氏雀麦等 3种近缘或远缘属间禾草的原生质体进行对称融合。对融合再生细胞系进行形态比较、同工酶分析及 5SrDNA间隔序列差异分析 ,以鉴定其杂种性质。对部分杂种细胞系进行了染色体组成的分析 ,就体细胞杂种中小染色体及染色体断片的产生与异源核质的互作及亲缘远近的关系等问题进行了讨论。
|
| 关 键 词： | 普通小麦 不同亲缘禾草 体细胞杂交 杂种染色体组成 |
| 修稿时间： | 2000年9月20日 |
PRELIMINARY STUDY ON HYBRID CHROMOSOME COMPOSITION AND RELATIONSHIP IN SYMMETRIC HYBRIDIZATION BETWEEN TRITICUM AESTIVUM AND INTERGENERIC GRASSES |
| |
| XING Mei-qing,XIA Guang-min,CHEN Hui-min.PRELIMINARY STUDY ON HYBRID CHROMOSOME COMPOSITION AND RELATIONSHIP IN SYMMETRIC HYBRIDIZATION BETWEEN TRITICUM AESTIVUM AND INTERGENERIC GRASSES[J].Bulletin of Botanical Research,2001,21(1):74-78. |
| |
| Authors: | XING Mei-qing XIA Guang-min CHEN Hui-min |
| |
| Institution: | XING Mei-qing 1 XIA Guang-min 2 CHEN Hui-min 2 |
| |
| Abstract: | The protoplasts of three intergeneric grasses ( Psathyrostachys juncea, Bromus inermis and Bromus willdenowii Kunth ) were fused with wheat ( Triticum aestivum . Jinan 177 ) protoplasts by PEG method respectively . The fusion products of wheat and grasses were analyzed with morphological inspection, isozyme analysis, 5S rDNA spacer sequence analysis. Parts of hybrid clones were taken for chromosome examination. Different chromosome compositions in calli were found between the fusion combinations of different phylogenetical distance, while microchromosomes or chromosome fragments were always present. The reason for this result and the failure of plant regeneration in this experiment is discussed. |
| |
| Keywords: | Triticum aestivum Intergeneric grasses Symmetric somatic hybridization Hybrid chromosome composition |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
