未经抗病毒治疗慢乙肝患者YMDD变异检测及HBV基因分型

目的 :探讨未接受拉米呋啶及干扰素抗病毒治疗的慢性乙型肝炎患者中YMDD是否存在变异及HBV的基因亚型。方法 :采用PCR荧光分子信标探针检测技术及PCR微板核酸杂交 ELISA技术进行HBVYMDD变异株 (YIDD YVDD)及HBV基因分型检测。结果 :2 9例慢性乙肝患者HBVYMDD变异阳性 6例 ,阳性率为 2 0 6%。 5例变异阳性中 3例为YIDD阳性 ,2例为YVDD阳性 ,6例均为YMDD野生株和变异株同时存在 ;HBV基因亚型以C、B、D亚型为主 ,分别占41 3% ( 1 2 2 9)、2 4 1 % ( 7 2 9)、2 0 6% ( 6 2 9)。结论 :在未经抗病毒治疗的慢乙肝患者中存在YMDD变异株 ,临床上在应用拉米呋啶对慢性乙型肝炎患者进行抗病毒治疗前 ,应对患者是否存在YMDD变异加以重视 。

Detection of YMDD Resistant Mutation and HBV DNA Genetypes in some Lamivudine-untreated Chronic Hepatitis B

Objectve:To explore the possibility of YMDD mutation and investigate HBV DNA genetypes in the lamivudine untreated patients with chronic hepatitis B.Methods:HBV DNA genetypes,YMDD and mutants(YIDD/YVDD) were examined by PCR microplate hybridization ELISA and Molecular Beacon,respectively.Results:YMDD mutations were detected in six of 29 samples:YIDD in four samples and YVDD in twosamples.Gengtype C,B,D are main gengtypes in HBV DNA from 29 hepatitis B patients serum as following:C 41 3% 12/29),B 24 1%(7/29),D 20 6%(6/29).Conclusion:The results showed that YMDD mutants,like the YMDD wild\|type,are naturally present in the lamivudine\|untreated patients with chronic hepatitis B.