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Characterization of GmCaMK1, a member of a soybean calmodulin-binding receptor-like kinase family |
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| Authors: | DeFalco Thomas A Chiasson David Munro Kim Kaiser Brent N Snedden Wayne A |
| Institution: | a Department of Biology, Queen’s University, Kingston, Ontario, Canada, K7L 3N6 b School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus Urrbrae, SA 5064, Australia c Protein Function Discovery Facility, Queen’s University, Kingston, Ontario, Canada |
| Abstract: | Calmodulin(CaM)-regulated protein phosphorylation forms an important component of Ca2+ signaling in animals but is less understood in plants. We have identified a CaM-binding receptor-like kinase from soybean nodules, GmCaMK1, a homolog of Arabidopsis CRLK1. We delineated the CaM-binding domain (CaMBD) of GmCaMK1 to a 24-residue region near the C-terminus, which overlaps with the kinase domain. We have demonstrated that GmCaMK1 binds CaM with high affinity in a Ca2+-dependent manner. We showed that GmCaMK1 is expressed broadly across tissues and is enriched in roots and developing nodules. Finally, we examined the CaMBDs of the five-member GmCaMK family in soybean, and orthologs present across taxa.Structured summaryMINT-8051564: AtCRLK2 (uniprotkb:Q9LFV3) binds (MI:0407) to CaM (uniprotkb:P62199) by filter binding (MI:0049)MINT-8051416: GmCaMK3 (uniprotkb:C6ZRS6) binds (MI:0407) to CaM (uniprotkb:P62199) by filter binding (MI:0049)MINT-8051258: CaM (uniprotkb:P62199) and GmCaMK1 (genbank_protein_gi:223452504) bind (MI:0407) by isothermal titration calorimetry (MI:0065)MINT-8051400: GmCaMK2 (uniprotkb: C6ZRY5) binds (MI:0407) to CaM (uniprotkb:P62199) by filter binding (MI:0049)MINT-8051242, MINT-8051295, MINT-8051313, MINT-8051327, MINT-8051341, MINT-8051355: GmCaMK1 (genbank_protein_gi:223452504) binds (MI:0407) to CaM (uniprotkb:P62199) by filter binding (MI:0049)MINT-8051467: GmCaMK4 (uniprotkb: C6TIQ0) binds (MI:0407) to CaM (uniprotkb:P62199) by filter binding (MI:0049)MINT-8051276: CaM (uniprotkb:P62199) and GmCaMK1 (genbank_protein_gi:223452504) bind (MI:0407) by comigration in non denaturing gel electrophoresis (MI:0404)MINT-8051374: CaM (uniprotkb:P62199) and GmCaMK1 (genbank_protein_gi:223452504) bind (MI:0407) by mass spectrometry studies of complexes (MI:0069) |
| Keywords: | CaM calmodulin CaMBD CaM-binding domain CaMBP CaM-binding protein CaMK calmodulin-binding kinase CaMRLK CaM-binding RLK CBL calcineurin B-like protein CCaMK Ca2+ and Ca2+/CaM-depedent protein kinase CDPK calcium-dependent protein kinase CIPK CBL-interacting protein kinase CML calmodulin-like protein CRK CDPK-related kinase GmCaMK1 Glycine max CaM-binding RLK GST glutathione S-transferase HRP horseradish peroxidase ITC isothermal titration calorimetry MALDI matrix-assisted laser desorption/ionization Q-tof quantitative time-of-flight RLK receptor-like kinase qPCR quantitative real-time PCR |
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