H7, a protein kinase C inhibitor, increases the glutathione content of neuroblastoma cells. |
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Authors: | F J Romero J Llopis V Felipo M D Mi?ana J Romá S Grisolía |
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Institution: | Departament de Fisiologia, Universitat de València, Spain. |
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Abstract: | It is shown that the intracellular glutathione (GSH) concentration of neuroblastoma-2a cells in culture increases with a maximum at 24 h after starting treatment with 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7), an inhibitor of protein kinase C (PKC). Other inhibitors of this and other protein kinases, e.g. sphingosine, staurosporine, and HA 1004, at the concentrations tested, had a less marked or negligible effect on intracellular GSH concentration. 12-O-Tetradecanoylphorbol-13-acetate (TPA) was also tested and showed no significant effect 24 h after addition. |
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