Influence of ovule perforation,plant growth regulators,andl-glutamine on in vitro growth of immature peach embryos |
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Authors: | Alberto C Q Pinto David H Byrne Suzanne M Dethier Rogers |
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Institution: | (1) EMBRAPA/CPAC, P.O. Box 700.023, Brasilia-DF, Brazil;(2) Dept. Hort. Sci., Texas A&M University, 77843 College Station, Texas |
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Abstract: | Summary Ovule perforation technique and media components (plant growth regulators andl-glutamine) were tested on in vitro growth of immature (<3 mm) embryos of “Springcrest” and “Earligrande” peaches. Ovule perforation
was 2 to 4 times more effective in promoting embryo growth than leaving ovules intact.l-Glutamine (400 mg·liter−1) promoted an increase in growth but could not be used with indole-acetic acid plus kinetin because an antagonistic effect
on embryo growth occurred. The use of these exogenous plant growth regulators did not increase embryo growth over in vivo
growth. |
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Keywords: | embryo rescue plant growth regulators in ovulo embryo culture l-glutamine" target="_blank">l-glutamine ovule culture peach |
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