Oestradiol, a new hapten for detecting nucleic acid sequences by FISH |
| |
| Authors: | Mariëtte P C van de Corput Roeland W Dirks Wouter W Wiegant Joop Wiegant Klaus Mühlegger A K Raap |
| |
| Institution: | (1) Department of Molecular Cell Biology, Laboratory for Cytochemistry and Cytometry, Leiden University, Wassenaarseweg 72, NL-2333 AL Leiden, The Netherlands Tel. +3171-5276187; Fax +3171-5276180 e-mail A.K. Raap@cytochemistry.medfac.leidenuniv.nl, NL;(2) Boehringer Mannheim, GmbH Werk Penzberg, Nonnenwald 2, D-82372 Penzberg, Germany, DE |
| |
| Abstract: | Oestradiol has been conjugated to allylamine-dUTP with an 11-atom spacer to allow enzymatic incorporation of the label into
DNA sequences. In a comparative DNA and mRNA FISH study we have used DNA probes that were either labelled with digoxigenin,
biotin or oestradiol. Results show that oestradiol-labelled probes can detect DNA and RNA sequences in FISH equally well as
digoxigenin- and biotin-labelled probes. Further, no crossreactivity between the various hapten-specific antibodies and the
three haptens were observed. Binding of the rabbit anti-oestradiol antibody to endogenous oestrogen in various tissues was
not observed under the conditions tested. In view of the increasing demands for multi-colour DNA and mRNA FISH applications,
oestradiol is a welcome addition to the collection of haptens employed in FISH.
Accepted 20 June 1997 |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
