移植神经营养素-4基因工程细胞对脊髓前角神经元的保护作用

用重组逆转录病毒介导的体外神经营养素－４（ＮＴ－４）基因转移的方法,制备高表达ＮＴ－４基因工程细胞并移植大鼠坐骨神经离断处,尼氏染色和ＣｈＥ染色的结果表明,移植ＮＴ－４基因工程细胞对外周神经损伤所造成的运动神经元的退变有显著的改善作用,而且这种作用可以维持三个月。

Protection of spinal motorneurons of section sciatic nerve by transplantation of NT-4 genetically expression cells into the side of section

The NT4 genetically engineered cells were made by infecting L6TG cell(a rat myoblast cell line) in vitro with a retroviral vector pN2A containing the rat NT4 cDNA. The bioactivities were determined by bioassay of PC12 cell survival rate. The rat with left sciatic nerve transaction was used as a model for treatment by implanting NT4 genetically modified cell. The condition of motorneurons was assessed by Nissl stain and ChE stain. The results showed that: (1) the percentage of surviving Nisslstained neurons on the lessened side of NT4( ) grafts significantly increased as compared to that with NT4(-) grafts 23 weeks and 3 months after sciatic nerve transaction, and (2) the grafted cells produced significant increase in the positive ChE stained area after sciatic nerve transaction in 13 weeks. Our observations indicate that adult motor neurons are still able to respond to neurotrophic factors and they may require the factors for survival.