Immunodiagnosis of clonorchiasis using a recombinant antigen |
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Authors: | Tai-Soon Yong Hye-Jin Yang Soon-Jung Park Yu-Kyoung Kim Du-Ho Lee and Sang-Mi Lee |
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Institution: | Department of Parasitology, Yonsei University College of Medicine, Seoul 120-752, Korea. |
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Abstract: | A cDNA expression library of Clonorchis sinensis adult worm was constructed, and screened out immunologically. One clone, pBCs31, was selected in view of its predominant reactivity with an experimentally infected rabbit serum. Recombinant C. sinensis antigen with 28 kDa as a β-galactosidase fusion protein produced in Escherichia coli was identified by immunoblot analysis. The cloned gene was composed of 16 copies of a 30 base pair repeat and an additional 320 bases. The deduced amino acid sequence of the tandem repeat was AQPPKSGDGG. On RNA slot blot analysis. C. sinensis adult worm RNA showed a positive reaction with the cloned gene. Enzyme-linked immunosorbent assay using a purified recombinant antigen of pBCs31 showed high specificity for diagnosis of clonorchiasis. |
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Keywords: | Clonorchis sinensis immunodiagnosis recombinant antigen repetitive sequence |
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