| HBV preS/S基因克隆及其在酿酒酵母中的表达 |
| |
| 引用本文： | 朱卫峰,郑文岭,詹万雷,马文丽.HBV preS/S基因克隆及其在酿酒酵母中的表达[J].中国生物工程杂志,2005,25(Z1):235-238. |
| |
| 作者姓名： | 朱卫峰 郑文岭 詹万雷 马文丽 |
| |
| 作者单位： | 1. 华南理工大学食品与生物工程学院, 广州 510640;
2. 广州军区总医院医学实验中心, 广州 510010;
3. 华南基因组研究中心, 广州 510860;
4. 南方医科大学基因工程研究所, 广州 510515 |
| |
| 基金项目： | 国家自然科学基金资助项目(36890044) |
| |
| 摘 要： | 目的:探索利用酿酒酵母系统表达乙型肝炎病毒(HBV)preS／S基因。方法:利用PCR技 术,以HBV病毒DNA为模板,体外扩增HBV preS／S基因。然后构建重组表达载体pESC-preS／S。 用LiAc法转化酿酒酵母YPH50,选取重组菌进行培养,并诱导表达外源蛋白。提取蛋白浓缩后 进行SDS-PAGE分析,并经Western blot分析鉴定。结果:实验结果表明重组菌能够表达HBV preS／S蛋白。结论:利用酿酒酵母系统可成功表达HBV preS／S基因,为制备新型预防性疫苗提供 条件。
|
| 关 键 词： | preS基因 酵母表达系统 蛋白表达 |
| 收稿时间： | 2004-10-27 |
Research on the HBV preS/S gene expression in S. cerevisiae System |
| |
| ZHU Wei-feng ZHENG Wen-ling ZHAN Wan-lei MA Wen-li College of Food Biotechnology,Southern China University of Science and Technology Guangzhou ,China Liu Hua Qiao Hospital,Dept of Medical Research Guangzhou ,China Southern China Genomics Research Center Guangzhou ,China Institute of Molecular Biology,Nanfang Medical University,Guangzhou ,China.Research on the HBV preS/S gene expression in S. cerevisiae System[J].China Biotechnology,2005,25(Z1):235-238. |
| |
| Authors: | ZHU Wei-feng ZHENG Wen-ling ZHAN Wan-lei MA Wen-li College of Food Biotechnology Southern China University of Science and Technology Guangzhou China Liu Hua Qiao Hospital Dept of Medical Research Guangzhou China Southern China Genomics Research Center Guangzhou China Institute of Molecular Biology Nanfang Medical University Guangzhou China |
| |
| Institution: | ZHU Wei-feng ZHENG Wen-ling ZHAN Wan-lei MA Wen-li College of Food Biotechnology,Southern China University of Science and Technology Guangzhou 510640,China Liu Hua Qiao Hospital,Dept of Medical Research Guangzhou 510010,China Southern China Genomics Research Center Guangzhou 510860,China Institute of Molecular Biology,Nanfang Medical University,Guangzhou 510515,China |
| |
| Abstract: | Objective: To explore the HBV preS/S expression in the S. cerevisiae system. Method: HBV preS/S gene fragments were amplified from HBV DNA in vitro, using PCR techniques. The amplified preS/S gene fragments were incorporated into the expression vector pESC to construct the pESC-preS/S. LiAc method were used to transfer this construct into the strain of S . cerevisiae . Protein synthesis after induction was analyzed with SDS-PAGE and Western blot. Results: It was demonstrated that the recombinant yeast could express HBV preS/S protein. Conclusion: the S. cerevisiae system have been used successfully in our experiments to express the HBV preS/S gene, which provide a basis for further research and development of new HBV vaccine for hepatitis infections. |
| |
| Keywords: | PreS/S gene S cerevisiae Protein expression |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国生物工程杂志》浏览原始摘要信息 |
| 点击此处可从《中国生物工程杂志》下载免费的PDF全文 |
|
