The mouse adducin gene family: alternative splicing and chromosomal localization |
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Authors: | Sardha P Suriyapperuma Larissa Lozovatsky Steven L Ciciotte Luanne L Peters Diana M Gilligan |
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Institution: | (1) Yale University School of Medicine, 333 Cedar Street, WWW 403, New Haven, Connecticut 06510, USA, US;(2) The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA, US |
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Abstract: | Mouse cDNA sequences encoding α, β, and γ adducins were cloned from a mouse reticulocyte cDNA library. The purified clones
contain alternatively spliced exons from all three adducin genes. In the case of α and β, the inclusion of the alternatively
spliced exons results in truncated polypeptide isoforms (called α-2 and β-2). The mouse predicted amino acid sequences are
compared with published rat and human sequences. For completion of this comparison, cDNA encoding the rat β-1 carboxy terminus
was cloned by PCR. The carboxy terminal region containing MARCKS homology, calmodulin-binding region-2, and spectrin-actin-binding
site, is conserved among α-1, β-1, and γ-1 isoforms in mouse, rat, and humans. We also report here the localization of the
gene encoding γ adducin (Add3) to murine Chr 19, in a region that shows conserved synteny with human Chr 10.
Received: 1 June 1999 / Accepted: 25 August 1999 |
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