Growth inhibition and differentiation induction in murine erythroleukemia cells by 4-hydroxynonenal |
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| Authors: | Monica Rinaldi Giuseppina Barrera Paola Spinsanti Stefania Pizzimenti Silvia Anna Ciafr Paola Parella Maria Giulia Farace Emanuela Signori Mario Umberto Dianzani Vito Michele Fazio |
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| Affiliation: | a Laboratory of Molecular Medicine & Biotechnology, University Campus Bio-Medico, School of Medicine, Roma, Italyb Institute of Experimental Medicine, CNR, Roma, Italyc Department of Experimental Medicine and Oncology, University of Torino, Torino, Italyd Department of Experimental Medicine and Biochemical Science, University of Roma “Tor Vergata”, Roma, Italye Laboratory for Molecular Pathology and Gene Therapy, IRCCS H. Casa Sollievo della Sofferenza, Foggia, Italy |
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| Abstract: | 4-Hydroxynonenal (HNE) is one of the major end products of lipid peroxidation. Here we show that the exposure of murine erythroleukemia (MEL) cells to 1 μM HNE, for 10.5 h over 2 days, induces a differentiation comparable with that observed in cells exposed to DMSO for the whole experiment (7 days). The exposure of MEL cells for the same length of time demonstrates a higher degree of differentiation in HNE-treated than in DMSO-treated MEL cells. The protooncogene c-myc is down-modulated early, in HNE-induced MEL cells as well as in DMSO-treated cells. However, ornithine decarboxylase gene expression first increases and then decreases, during the lowering of the proliferation rate. These findings indicate that HNE, at a concentration physiologically found in many normal tissues and in the plasma, induces MEL cell differentiation by modulation of specific gene expression. |
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