Growth inhibition and differentiation induction in murine erythroleukemia cells by 4-hydroxynonenal |
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| Authors: | Monica Rinaldi Giuseppina Barrera Paola Spinsanti Stefania Pizzimenti Silvia Anna Ciafr Paola Parella Maria Giulia Farace Emanuela Signori Mario Umberto Dianzani Vito Michele Fazio |
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| Institution: |
a Laboratory of Molecular Medicine & Biotechnology, University Campus Bio-Medico, School of Medicine, Roma, Italy
b Institute of Experimental Medicine, CNR, Roma, Italy
c Department of Experimental Medicine and Oncology, University of Torino, Torino, Italy
d Department of Experimental Medicine and Biochemical Science, University of Roma “Tor Vergata”, Roma, Italy
e Laboratory for Molecular Pathology and Gene Therapy, IRCCS H. Casa Sollievo della Sofferenza, Foggia, Italy |
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| Abstract: | 4-Hydroxynonenal (HNE) is one of the major end products of lipid peroxidation. Here we show that the exposure of murine erythroleukemia (MEL) cells to 1 μM HNE, for 10.5 h over 2 days, induces a differentiation comparable with that observed in cells exposed to DMSO for the whole experiment (7 days). The exposure of MEL cells for the same length of time demonstrates a higher degree of differentiation in HNE-treated than in DMSO-treated MEL cells. The protooncogene c-myc is down-modulated early, in HNE-induced MEL cells as well as in DMSO-treated cells. However, ornithine decarboxylase gene expression first increases and then decreases, during the lowering of the proliferation rate. These findings indicate that HNE, at a concentration physiologically found in many normal tissues and in the plasma, induces MEL cell differentiation by modulation of specific gene expression. |
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