Nitroindoles as Universal Bases |
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| Authors: | D. Loakes F. Hill S. Linde D. M. Brown |
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| Affiliation: | 1. Medical Research Council, Laboratory of Molecular Biology , Hills Road, Cambridge, CB2 2QH;2. Department of Haematology , University of Cambridge , Hills Road, Cambridge, United Kingdom |
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| Abstract: | Abstract Nitroindoles behave as non-discriminatory bases when incorporated into oligodeoxynucleotides, without causing significant destabilisation of the duplex, and exhibit higher melting temperatures than 3-nitropyrrole. When 5-nitroindole and 3-nitropyrrole are incorporated into oligomers for use as primers for sequencing and the polymerase chain reaction, both give products but are not as effective as hypoxanthine. |
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