| Institution: | (1) Department of Animal Health, Institute for Tropical Medicine, 155 Nationalestraat, 2000 Antwerp, Belgium;(2) Laboratory for Thrombosis Research, IRC, KU Leuven Campus Kortrijk, Kortrijk, Belgium;(3) Laboratory of Molecular Plant Physiology and Biotechnology, Department of Biology, University of Antwerp, Antwerp, Belgium;(4) Institute for Agricultural and Fisheries Research, Animal Department, Oostende, Belgium;(5) Laboratory of Ecophysiology, Biochemistry and Toxicology, Department of Biology, University of Antwerp, Antwerp, Belgium;(6) Department of Biochemistry and Molecular Biology, The University of British Columbia, Vancouver, Canada;(7) Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, Antwerp, Belgium |
| Abstract: | The effects of various environmental factors such as pH (5, 6, 7, 8 and 9), temperature (30, 37 and 40°C) and rotational speed
(150, 200 and 250 rpm) on the growth and the hepatitis B core antigen (HBcAg) production ofEscherichia coli W3110IQ were examined in the present study. The highest growth rate is achieved at PH 7, 37°C and at a rotational speed of
250 rpm which is 0.927 h−1. The effect of pH on cell growth is more substantial compared to other parameters; it recorded a 123% different between the
highest growth rate (0.927 h−1) at pH 7 and lowest growth at pH 5. The highest protein yield is achieved at pH 9, rotational speed of 250 rpm and 40°C.
The yield of protein at pH 7 is 154% higher compared to the lowest yield achieved at pH 5. There is about 28% different of
the protein yield for theE. coli cultivated at 250 rpm compared to that at 150 rpm which has the lowest HBcAg yield. The yield of protein at 40°C is 38% higher
compared to the lowest yield achieved, at 30°C. |
