Isolation and some properties of mammalian hepatic membrane lectins |
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Authors: | N Ali A Salahuddin |
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Institution: | Interdisciplinary Biotechnology Unit, Aligarh Muslim University, India. |
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Abstract: | Membrane lectins were isolated from sheep, goat, and buffalo liver by chromatography on an asialofetuin (ASF)-Sepharose 4B column. The lectins moved as a single protein band in SDS-PAGE with molecular masses of 42, 54 and 50 kDa, respectively, for sheep, goat and buffalo lectins. The molecular masses remained unchanged in 0.2 M 2-mercaptoethanol. As judged from the inhibition of binding of the lectin to ASF gel, the three lectins were beta-galactoside-specific. Sheep, goat and buffalo lectins were found to be sialoglycoproteins containing 18.6, 27 and 38.8 mol/mol lectin of neutral hexose, respectively; the corresponding values for the sialic acid content being 5.3, 8.7 and 11.8 mol/mol lectin. Thus goat and buffalo lectins are physico-chemically different from many mammalian hepatic lectins described so far. |
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