广西HBsAg阳性母亲的胎儿肝、肾细胞中乙肝病毒DNA存在状态的研究

采用核酸分子杂交Ｓｏｕｔｈｅｒｎ印迹法，以３２Ｐ标记的ＨＢＶＤＮＡ为探针，检测ＨＢｓＡｇ阳性母亲引产的４０例胎儿的肝、肾组织。结果有２例胎肝和１例胎肾细胞ＤＮＡ出现大于３．２ｋｂ的杂交带，表明ＨＢＶＤＮＡ已处于整合状态。胎肾细胞基因组中查出ＨＢＶＤＮＡ整合为首次报道。

Study on Status of HBV DNA in Genomes of Liver and kidney Cell of Fetuses Aborted from HBsAg Positive Mothers in Guangxi

This paper reports the detection results of HBV DNA sequences state in liver and kidney cell genomes of fetuses aborted from HBsAg positive mothers by Southern blot hybridization technique and cloned HBV DNA as probe labelled with 32P. In 40 fetuses,specific hybridization bands corresponding to high molecular weight DNA over 3. 2 kb of HBV DNA were observed in cellular DNAs extracted from 2 cases of fetal liver and I case of fetal kidney tissues after digestion by EcoRI and hybridization with 32P HBV DNA probe. The results showed that HBV DNA sequences could integrate into hepatocyte and kidney cell genomes. The integration of HBV DNA in cell genomes might occur randomly since the integrating bands in fetal celluar DNA did not appear at same position. HBV DNA sequence was not found in the remaining tissue samples. None of free HBV DNA hybridization band could be detected in the cellular DNAs extracted from all fetal organs studied.