菜籽粕脱毒液中硫代葡萄糖苷提取工艺的研究

本文用两步萃取法从菜籽粕脱毒液中提取硫代葡萄糖苷，以3，5-二硝基水杨酸比色法测定硫代葡萄糖苷含量，采用单因素试验法，分别考察可能影响硫代葡萄糖苷提取效果的各种因素，确定了最佳工艺条件：第一步萃取剂为异辛烷，母液与异辛烷体积比为2：1，搅拌时间为30min，异辛烷萃取次数为2次，异辛烷单次萃取率达71．2％。硫代葡萄糖苷收率为83．7％。

Study on Extracting Procedure of Glucosinolates from Rapeseed Meal Detoxifying Solution

This paper discussed the extraction of glucosinolates from rapeseed meal detoxifying solution. The content of glucosinolates was determined by 3,5-dinitrosalicylic acid colorimiay and the various factors responsible for the effect of extraction of glucosinolates were investigated respectively by single factor method. The most suitable extraction conditions were as follows:in the first step,where the volume ratio of mother solution to extractant, which was isooctane,was 2: 1, extraction yield reached 71.2% in the first extraction by isooctane. In this step,there were two extractions, each of which lasted for 30 min. The total recovery of glucosinolates was 83.7%.