Upstream regulatory regions from the maize Sh1 promoter confer tissue-specific expression of the ,-glucuronidase gene in tomato |
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| Authors: | M-A D'Aoust B Nguyen-Quoc V-Q Le S Yelle |
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| Institution: | (1) Centre de recherche en horticulture, Pavillon de l'Envirotron, Université Laval, Sainte-Foy, Québec, G1K 7P4, Canada e-mail: nguyenb@plg.ulaval.ca Fax: +1-418-6567871, CA;(2) Centre de recherche en biologie forestière, Pavillon Charles-Eugène Marchand, Université Laval, Sainte-Foy, Québec, G1K 7P4, Canada, CA |
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| Abstract: | A promoter fusion (Sh35) combining upstream regulatory regions from the maize Sh1 promoter with a truncated 35S promoter, Δ9035 (–90 to +8) has been compared with the original Sh1 promoter for its capacity to promote expression of the β-glucuronidase (GUS) gene in stably transformed tomato plants. For both promoters, very faint GUS expression was detected
in the vegetative tissues, and no expression was detected in the fruit pericarp tissues. However, in the seed, Sh1 promoted low GUS expression but Sh35 directed 25-fold higher GUS expression. For both constructs, the profile of GUS expression
was similar to that of endogenous sucrose synthase activity, but maximal GUS activity was reached 15 days after the peak of
sucrose synthase activity.
Received: 20 October 1998 / Revision received: 1 December 1998 / Accepted: 14 December 1998 |
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| Keywords: | Maize Promoter Shrunken-1 Sucrose synthase Tomato |
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