Evaluation of hybridization conditions for spotted oligonucleotide-based DNA microarrays |
| |
| Authors: | Mong-Hsun Tsai Hailing Yan Xi Chen G. V. R. Chandramouli Shuping Zhao Deborah Coffin C. Norman Coleman James B. Mitchell Eric Y. Chuang |
| |
| Affiliation: | (1) Radiation Oncology Sciences Program, Radiation Oncology Branch, Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 8717 Grovemont Cr., Room 109J, 20878 Gaithersburg, MD |
| |
| Abstract: | We compared different hybridization conditions of oligonucleotide-based DNA microarray to acquire optimized and reliable microarray data. Several parameters were evaluated at different hybridization conditions, including signal-to-background (S:B) ratios, signal dynamic range, usable spots, and reproducibility. Statistical analysis showed that better results were obtained when spotted, presynthesized long oligonucleotide arrays were blocked with succinic anhydride and hybridized at 42°C in the presence of 50% formamide. |
| |
| Keywords: | Gene expression hybridization conditions oligonucleotide microarrays |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
