Extraction and quantitation of astaxanthin from Phaffia rhodozyma |
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Authors: | J James Sedmak Deepthi K Weerasinghe Setsuko O Jolly |
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Institution: | (1) Molecular Biology Department, Universal Foods Corporation, 6143 N. 60th Street, 53218 Milwaukee, Wisconsin, USA |
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Abstract: | Summary The rapid, quantitative release of astaxanthin and other carotenoids from the yeast Phaffia
rhodozyma is described. Hashed cells are ruptured with dimethylsulfoxide (DMSO) and carotenoids extracted into an organic solvent. Extraction and spectrophotometric quantitation of total carotenoids is rapid, reproducible and only small volumes (0.1–2 ml) of culture are required. HPLC analysis in normal phase silica gel column indicates that astaxanthin comprises 65–95% of the total pigmented carotenoids of P.
rhodozyma. |
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