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Use of gene fusions to study the expression of fnr, the regulatory gene of anaerobic electron transfer in Escherichia coli
Authors:Marie-Claire Pascal  Violaine Bonnefoy  Michel Fons  Marc Chippaux
Affiliation:Laboratoire de Chimie Bactérienne, C.N.R.S. 31, chemin J. Aiguier, P.O. Box 71, 13277 Marseille Cedex 9, France
Abstract:
Abstract Fusions between fnr , the regulatory gene for anaerobic electron transfer, and lacZ were obtained by insertion of Mu d II1734 bacteriophage in the fnr gene cloned in a plasmid. After transfer onto the chromosome, study of the fusion showed that the expression of fnr is independent of anaerobiosis, negatively regulated by its own product and partly positively controlled by cyclic AMP.
Keywords:Gene fusions    autoregulation    fnr    Escherichia coli    anaerobic electron transport
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