钙与植物乙烯反应的关系研究

研究了Ca2+ 对番茄(Lycopersicon esculentum Mill cv. Lichun)黄化幼苗乙烯反应的影响.通过测定不同Ca2+ 浓度条件下番茄黄化幼苗的"三重反应"、内源乙烯释放量、乙烯受体基因NEVER-RIPE(NR)表达量及胞内CaM含量的变化,结果发现,随着培养基中Ca2+ 浓度从0 mmol/L增加到3.8 mmol/L,番茄黄化幼苗的"三重反应"表型明显增强,内源乙烯释放量、NR基因的表达量及胞内CaM的含量都有不同程度的增加;当Ca2+ 浓度由3.8 mmol/L进一步增加到10 mmol/L时,番茄黄化幼苗"三重反应"表型受到抑制,内源乙烯释放量、 NR基因的表达量及胞内CaM的含量都有所下降.因此,Ca2+ 对番茄黄化幼苗"三重反应"的影响与Ca2+ 调节内源乙烯合成和乙烯受体基因的表达有关,而且Ca2+ 可能是通过CaM含量的变化来调节乙烯作用的.

Relationship Between Ca2+ and Plant Ethylene Response

The relationship between Ca2+ and ethylene response was investigated through analyzing the effect of Ca2+ on the response to ethylene in etiolated tomato (Lycopersicon esculentum Mill cv. Lichun) seedling grown in darkness. When the etiolated tomato seedlings were treated with different concentrations of Ca2+, the “triple response" phenotype, ethylene production, the expression of ethylene receptor gene NEVER RIPE (NR) and the content of cytosolic CaM were determined. With the concentration of Ca2+ in the culture medium increasing from 0 mmol/L to 3.8 mmol/L, the “triple response" phenotype of etiolated tomato seedling was correspondingly strengthened; meanwhile the ethylene production, the amount of NR gene expression and the concentration of CaM increased respectively. However, when the concentration of Ca2+ was increased from 3.8 mmol/L to 10 mmol/L, the phenotype of “triple response”, ethylene production, NR gene expression, and the CaM content didn't increase further, but decreased consequently. The results indicated that the effect of Ca2+ on the ethylene triple response in etiolated tomato seedling was relevant to ethylene biosynthesis and ethylene receptor gene expression which were influenced by applied Ca2+, and these effects might be mediated through the change of CaM concentration in plant cell.