The chicken HPRT gene: a counter selectable marker for the DT40 cell line |
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| Authors: | Fukagawa T Hayward N Yang J Azzalin C Griffin D Stewart A F Brown W |
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| Institution: | Biochemistry Department, Oxford University, South Parks Road, Oxford OX1 3QU, UK, Brunel University, Uxbridge, Middlesex UB8 3PH, UK. |
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| Abstract: | We describe the cloning, characterisation and chromosomal mapping of the chicken hprt gene together with the construction of two counter selectable hprt-/- DT40 derived cell lines. One of these cell lines contains a stably integrated gene encoding a conditionally active cre recombinase and thus allows efficient manipulation of targeted loci by site-specific recombination. These cell lines will enhance the utility of the hyper-recombinogenic DT40 cell line as a system for the genetic analysis of cell autonomous functions in vertebrates and as a tool for mammalian chromosome engineering. |
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