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Immunodetection of pectin and arabinogalactan protein epitopes during pollen exine formation of Beta vulgaris L.
Authors:Anna Majewska-Sawka  Maria Isabel Rodriguez-Garcia
Affiliation:(1) Institute of Plant Breeding and Acclimatization, Bydgoszcz;(2) Departamento de Bioquimica, Biologia Celular y Molecular de Plantas, Estación Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Granada
Abstract:
Summary. We present the results of ultrastructural and immunocytochemical studies of sugar beet microsporocytes during the developmental phase that begins with the first meiotic metaphase and ends with the formation of young tetrads. The most prominent feature noted during this period of microsporogenesis was the presence of numerous cisternae of endoplasmic reticulum which frequently lie perpendicular to the surface of the plasma membrane and eventually fuse to it. Microscopic observations have been combined with the detection of several carbohydrate epitopes representing pectins and arabinogalactan proteins in the primexine and incipient exine. Pectin domains that possess both low and highly methylesterified epitopes, as well as pectin side chains enriched in (1→4)-β-D-galactose residues, are deposited in this young microspore wall. The epitopes of arabinogalactan protein that bind to JIM13, JIM8, and LM2 antibodies are localised within the callose wall surrounding posttelophase tetrads. The possibility of endoplasmic-reticulum involvement in the synthesis, transport, or metabolism of several microspore wall compounds is discussed. Correspondence and reprints: Institute of Plant Breeding and Acclimatization, Powstańców Wielkopolskich 10, 85-090 Bydgoszcz, Poland.
Keywords:: Beta vulgaris   Callose   Endoplasmic reticulum   Exine   Pectin   Proteoglycan.
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