| 甘蓝型油菜BcNA1基因启动子在转基因烟草中对GUS基因表达的调控 |
| |
| 引用本文： | 石东乔,周奕华,万里红,刘桂珍,胡赞民,陈正华. 甘蓝型油菜BcNA1基因启动子在转基因烟草中对GUS基因表达的调控[J]. 植物生理与分子生物学学报, 2001, 27(4): 313-320 |
| |
| 作者姓名： | 石东乔 周奕华 万里红 刘桂珍 胡赞民 陈正华 |
| |
| 作者单位： | 中国科学院遗传研究所, |
| |
| 基金项目： | 国家自然科学基金资助（39770465）。 |
| |
| 摘 要： | 通过PCR扩增，从甘蓝型油菜（Brassica
|
| 关 键 词： | 油菜，BcNA1启动子，植物表达载体，农杆菌介导，烟草 |
| 修稿时间： | 2000-11-20 |
Expression of GUS Gene in Transgenic Tobacco Regulated by BcNA1 Promoter fromBrassica napus |
| |
| SHI Dong Qiao ZHOU Yi Hua WAN Li Hong LIU Gui Zhen HU Zan Min CHEN Zheng Hua. Expression of GUS Gene in Transgenic Tobacco Regulated by BcNA1 Promoter fromBrassica napus[J]. Journal Of Plant Physiology and Molecular Biology, 2001, 27(4): 313-320 |
| |
| Authors: | SHI Dong Qiao ZHOU Yi Hua WAN Li Hong LIU Gui Zhen HU Zan Min CHEN Zheng Hua |
| |
| Abstract: | The upstream regulatory region of a seed specific gene was separated from Brassica napus cv. H165 genomic DNA by polymerase chain reaction. Plant expression vector was constructed by ligating this promoter with GUS gene and transferred into tobacco. GUS analysis made on the transgenic plants indicated that the promoter could drive the expression of GUS gene seed specifically and the activity of GUS changed with the development of seeds. The results of GUS assay also suggested that the genetic transmission of the BcNA1 promoter conforms to Mendelian laws. |
| |
| Keywords: | Brassica napus BcNA1 promoter plant expression vector Agrobacterium mediation Nicotiana tabacum |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
