Ethylene biosynthesis in a chilling-sensitive<Emphasis Type="Italic">Arabidopsis</Emphasis> mutant,<Emphasis Type="Italic">chs4-2</Emphasis> |
| |
Authors: | Sun-A?Kim Seong-Ki?Kim Peter?B?Kaufman Email author" target="_blank">June?Seung?LeeEmail author Email author" target="_blank">Soo?Chul?ChangEmail author |
| |
Institution: | (1) Department of Biological Science, Ewha Womans University, 120-750 Seoul, Korea;(2) Department of Life Science, Chung-Ang University, 156-756 Seoul, Korea;(3) Department of Molecular, Cellular, and Developmental Biology, University of Michigan, 48109-1048 Ann Arbor, Ml, USA;(4) University College, Yonsei University, 120-749 Seoul, Korea |
| |
Abstract: | We investigated chilling-induced changes in ethylene levels in Arabidopsis to find plants with distinct patterns of ethylene
production in the cold-related biosynthetic pathway. The sensitive mutants identified here includedchs1-2,chs4-2, andchs6-2. Among these, plants of thechs4-2 mutant produced more ethylene than did the wild type after both were transferred from 4°C or 10°C to 22°C. This mutant also
showed less freezing tolerance and more electrolyte leakage than the wild-type plants. Our results suggest a relationship
between ethylene biosynthesis and chilling sensitivity in the mutant To determine which of the enzymes involved in ethylene
biosynthesis were induced by chilling, we tested the activities of ACC synthase and ACC oxidase in both mutant and wild-type
plants, and found greater activity by ACC synthase as well as a higher ACC content in the mutants after all the plants were
transferred from 10°C to 22°C. However, ACC oxidase activity did not differ between mutant and wild-type plants in response
to chilling treatment Therefore, we conclude thatchs4-2 mutants produce more ethylene than do other mutants or the wild type during their recovery from chilling conditions. Furthermore,
we believe that ACC synthase is the key enzyme involved in this response. |
| |
Keywords: | ACC synthase Arabidopsis chilling chs mutant ethylene |
本文献已被 SpringerLink 等数据库收录! |
|