骨髓间充质干细胞治疗脑梗死

目的:研究骨髓间充质干细胞(MSC)对大鼠脑缺血再灌注损伤的治疗机制。方法:20只Wistar大鼠随机分为对照组和MSC治疗组。应用GFP阳性MSC,再灌注1d后经尾静脉注射MSC(1×106),对照组则注射PBS。采用线栓法建立脑缺血再灌注模型。术后每天由双盲于试验组的研究人员应用爬杆计分法评定大鼠神经功能。缺血2h再灌注8d取脑组织,用免疫组织化学方法检测脑组织中bFGF的表达。结果:MSC治疗组大鼠的神经功能缺损评分明显低于手术组和对照组(P<0.05)。MSC治疗组缺血侧缺血周边区脑组织中观察到GFP阳性与bFGF免疫组化染色阳性细胞。结论:经尾静脉给予的MSC可促进脑缺血再灌注大鼠的运动功能恢复;bFGF表达升高,可能是MSC脑保护作用机制之一。

Study on Bone Marrow Mesenchymal Stem Cells in the Treatment of Cerebral Ischemia- Reperfusion Injury in Rats

Objective: To investigate the mechanism of mesenchymal stem cells(MSCs) treating cerebral Ischemia-reperfusion injury of wistar rats.Methods: 20 Wistar rats were randomly divided into control group,MSC-treated group.One day after the reperfusion,1 x10~6 MSC were injected via tail vein and the same dose of the PBS was given to the control group.Cerebral ischemia reperfusion model was established with the suture method.8 days' reperfusion was given to the rats,after 2 hours of middle cerebral artery occlusion(MCAO),whose cerebral tissues were obtained.The neurological deficits of the rats were evaluated by the blind investigator of the research every day with the modified neurological Severity Score Points.The MSC were detected by Laser Scanning Confocal Microscopy and the basic fibroblast growth factor(bFGF) expression was detected by immuno-histochemistry.Results: The score of neurological impairment in the MSC-treated group was significantly lower than that in the control group(P<0.05),with GFP and bFGF immunochemical stain positive cells around the ischemic brain.Conclusion: MSC given via tail vein can promote motor function recovery of cerebral Ischemia reperfusion rat.The increase of expression may be one of the MSC's neuro-protective mechanisms.