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Chromosome aberrations caused by the effect of ultrasound in the meristematic cells ofvicia faba
Authors:Jana Šlotová  Zdenék Kaepfel  Ivo Hrazdira
Institution:1. Institute of Biophysics, Czechoslovak Academy of Sciences, Královopolská 135, Brno
Abstract:In our present work the formation of chromosome aberrations has been studied in dependence on the tima interval between sonication and fixation of the primary root tips of Vicia faba. Maximum occurrence of aberrations was recorded immediately after sonication. The results of our experiments pointed to the fact that the frequency of the induced changes was independent on the sonic waves intensity within the range of 0-2—3-0 W/cm2 and on ultrasond treatment duration within the range of 1—20 min. Studies of the distribution of chromosome abnormalities caused by ultrasound between the large and small chromosomes of theVicia faba meristematic cells in various time intervals showed that the frequency of the aberrations in both chromosome groups was proportional to its total metaphase lengths. Analysis of the type of aberrations observed in various time intervals after sonication indicated the simultaneous formation of chromosome and chromatide abnormalities.
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