Phylogenetic relationships of Cyprinidae (Teleostei: Cypriniformes) inferred from the partial S6K1 gene sequences and implication of indel sites in intron 1 |
| |
Authors: | KONG XiangHui WANG XuZhen GAN XiaoNi LI JunBing & HE ShunPing |
| |
Institution: | (1) Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China;(2) College of Life Science, Henan Normal University, Xinxiang, 453007, China |
| |
Abstract: | The family Cyprinidae is widely distributed in East Asia, and has the important phylogenetic significance in the fish evolution.
In this study, the 5′ end partial sequences (containing exon 1, exon 2 and indel 1) of S6K1 gene were obtained from 30 representative species in Cyprinidae and outgroup using PCR amplification and sequencing. The
phylogenetic relationships of Cyprinidae were reconstructed with neighbor joining (NJ), maximum parsimony (MP), maximum likelihood
(ML), and Bayesian methods. Myxocyprinus asiaticus (Catostomidae) was assigned to the outgroup taxon. Similar phylogenetic relationships within the family Cyprinidae were achieved
with the four analyses. Leuciscini and Barbini were monophyletic lineages respectively with the high nodal supports. Leuciscini
comprises Hypophthalmichthyinae, Xenocyprinae, Cultrinae, Gobioninae, Acheilognathinae and East Asian species of Leuciscinae
and Danioninae. Monophyly of East Asian clade was supported with high nodal support. Barbini comprises Schizothoracinae, Barbinae,
Cyprininae and Labeoninae. The monophyletic lineage consisting of Danio rerio, D. myersi, and Rasbora trilineata was basal in the tree. In addition, the large fragment indels in intron 1 were analyzed to improve the understanding of Cyprinidae
relationships. The results showed that the large fragment indels were correlated with the relations among species. Some conserved
regions in intron 1 were thought to be involved in the functional regulation. However, no correlation was found between sequence
variations and species characteristic size.
Supported by the Development Plan of the State Key Fundamental Research of China (Grant No. 2004CB117402), the National Natural
Science Foundation of China (Grant No. 30530120) and Chinese Postdoctoral Science Foundation (Grant No. 2005037684) |
| |
Keywords: | Cyprinidae S6K1 gene phylogenetic relationships intron |
本文献已被 万方数据 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学:生命科学英文版》浏览原始摘要信息 |
| 点击此处可从《中国科学:生命科学英文版》下载免费的PDF全文 |
|