Stable expression of human growth hormone over 50 generations in transgenic insect larvae |
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| Authors: | Maria Markaki Dubravka Drabek Ioannis Livadaras Roger K Craig Frank Grosveld Charalambos Savakis |
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| Institution: | (1) Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, PO Box 1385, Heraklion , 71110, Greece;(2) Department of Cell Biology, Erasmus MC, PO Box 1738, 3000DR Rotterdam, The Netherlands;(3) Minos BioSystems Ltd, Jubilee House Farm, Sandbach, CW11 2XB, UK;(4) Division of Medical Sciences, Medical School, University of Crete, Heraklion, Crete, Greece |
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| Abstract: | Developments in insect transgenesis using transposons combined with available mass rearing technology for insects such as
the Medfly, Ceratitis capitata, provide opportunity for the production of protein for industrial, agricultural and healthcare purposes on a very large scale.
In this study, we report the germ-line transformation and expression of a cDNA encoding human growth hormone (hGH) in transgenic
Drosophila using the Minos transposon. Production and secretion of a bioactive hGH into the haemolymph of transgenic larvae was demonstrated by immunoblot
analysis, ELISA and a proliferation bioassay. Stable expression of hGH was observed over 50 generations. The results indicate
that mass reared transgenic diptera with a rapid period of larval growth could provide cost effective production systems for
the manufacture of therapeutic and other high value proteins. |
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| Keywords: | Transgenic insect larvae High value proteins hGH Minos transposable element Stable germ line transformation Mass rearing |
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