| 对5个S180克隆细胞株RAPD特征的研究 |
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| 引用本文： | 贺文,张焕玲,王俊霞,刘福英.对5个S180克隆细胞株RAPD特征的研究[J].中国实验动物学报,2005,13(3):173-175. |
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| 作者姓名： | 贺文 张焕玲 王俊霞 刘福英 |
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| 作者单位： | 河北医科大学实验动物学部,石家庄,050017 |
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| 基金项目： | 河北省科学技术厅基金项目(03276196D-48)。 |
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| 摘 要： | 目的运用随机扩增多态性DNA(RAPD)技术建立S180的5个克隆细胞株的特异性图谱。方法运用23条引物对S180-S2D9、S180-S2D7、S180-S1F11、S180-S1H10、S180-S1B115个克隆细胞株的基因组DNA进行RAPD-PCR扩增,以琼脂糖电泳观察结果。结果筛选出3条在各克隆株扩增产物间有差异的引物。结论5个S180克隆细胞株的RAPD特征有明显区别。
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| 关 键 词： | 随机扩增多态DNA技术 S180 克隆细胞 DNA引物 |
| 文章编号： | 1005-4847(2005)03-0173-03 |
| 修稿时间： | 2004年10月13 |
RAPD Characterization of 5 Clonal Cell Strains from S180 |
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| HE Wen,ZHANG Huan-ling,WANG Jun-xia,LIU Fu-ying.RAPD Characterization of 5 Clonal Cell Strains from S180[J].Acta Laboratorium Animalis Scientia Sinica,2005,13(3):173-175. |
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| Authors: | HE Wen ZHANG Huan-ling WANG Jun-xia LIU Fu-ying |
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| Institution: | HE Wen,ZHANG Huan-ling,WANG Jun-xia,LIU Fu-ying* |
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| Abstract: | Objective To set up differential specific maps of 5 clonal cell strains from S180 with RAPD. Methods The Genomic DNA of the 5 clonal strains from S180 including S2D9, S2D7, S1F11, S1H10 and S1B11 was amplified with RAPD-PCR using 23 single primers. The PCR results were assessed by electrophoresis on agarose gel. Results 3 of the 23 single primers were found to be different between amplified products from different clonal strains.Conclusion RAPD characteristics of the 5 clonal cell strains from S180 are apparently different. |
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| Keywords: | Random Amplified Polymorphic DNA technique S180 Clonal cells DNA primers |
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