Effects of temperature, and availability of nitrogen and phosphorus on the abundance of Anabaena and Microcystis in Lake Biwa, Japan: an experimental approach |
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Authors: | C Nalewajko T P Murphy |
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Institution: | (1) Life Sciences Division, University of Toronto at Scarborough, 1265 Military Trail, Scarborough, Ontario M1C 1A4, Canada Tel. +1-416-287-7427; Fax +1-416-287-7642 e-mail: nalewajko@scar.utoront.ca, CA;(2) Water Issues Division, National Water Research Institute, Ontario, Canada, CA |
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Abstract: | Under optimal nutrient conditions, both Microcystis sp. and Anabaena sp. isolated from Lake Biwa grew optimally at 28–32°C but differed in maximal growth rates, phosphate uptake kinetics, maximal
phosphorus quotas, and growth responses to nitrogen and phosphorus limitation. The maximal growth rates of Microcystis and Anabaena were 1.6 and 1.25 divisions day−1, respectively. With phosphate and nitrate in the growth-limiting range, the growth of Microcystis was optimal at an N : P ratio of 100 : 1 (by weight) and declined at lower (nitrogen limitation) and higher (phosphorus limitation)
ratios. In contrast, Anabaena growth rates did not change at N : P ratios from 1000 : 1 to 10 : 1. Starting with cells containing the maximal phosphorus
quota, Microcystis growth in minus-phosphorus medium ceased in 7–9 days, compared with 12–13 days for Anabaena. The phosphate turnover time in cultures starved to their minimum cell quotas was 7.9 min for Microcystis and 0.6 min for Anabaena. Microcystis had a higher K
s (0.12 μg P l−1 10−6 cells) and lower V
max (9.63 μg P l−1 h−1 10−6 cells), than Anabaena (K
s 0.02 μg P l−1 h−1 10−6 cells; V
max 46.25 63 μg P l−1 h−1 10−6 cells), suggesting that Microcystis would not be able to grow well in phosphorus-limited waters. We conclude that in spite of the higher growth rate under ideal
conditions, Microcystis does not usually bloom in the North Basin because of low availability of phosphorus and nitrogen. Although Anabaena has an efficient phosphorus-uptake system, its main strategy for growth in low-phosphorus environments may depend on storage
of phosphorus during periods of abundant phosphorus supply, which are rare in the North Basin.
Received: July 31, 2000 / Accepted: October 18, 2000 |
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Keywords: | Anabaena Microcystis Lake Biwa Temperature Nitrogen and phosphorus ratios |
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