Two-stage culture for producing berberine by cell suspension and shoot cultures of <Emphasis Type="Italic">Berberis buxifolia</Emphasis> Lam |
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Authors: | María A Alvarez Natalia Fernandez Eraso Sandra I Pitta-Alvarez Patricia L Marconi |
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Institution: | (1) Instituto de Ciencia y Tecnología Dr César Milstein, CONICET, Saladillo, 2468 (C1440FFX), Ciudad de Buenos Aires, Argentina;(2) INGEBI, Obligado, 2490 (C1428), Ciudad de Buenos Aires, Argentina |
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Abstract: | In vitro cultures of Berberis buxifolia were established using thidiazuron (4.5, 23 and 45 mM) or picloram (4 and 40 mM) as plant growth regulators for sustaining
growth. For producing berberine, a two-stage culture was performed. In the first step, thidiazuron or picloram were used for
biomass production followed by the production stage where benzylaminopurine (4.4 mM) was added as a plant growth regulator.
Berberine yields (102 mg g−1 DW) and in vitro shoot cultures (200 mg g−1 DW) were significantly lower than those of whole plants in the field (416 mg g−1 DW). The highest productivity (0.18 mg 1−1 day−1) was attained using picloram (either 4 on 40 mM) in the first stage for producing biomass. |
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Keywords: | Berberine Berberis buxifolia Picloram Thidiazuron |
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