Screening of yeast strains capable of hyperproducing amylolytic enzymes |
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Authors: | Yong K Park Edna H Azuma |
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Institution: | (1) Faculdade de Engenharia de Alimentos (UNICAMP), Universidade Estadual de Campinas, Caixa postal 6121, Campinas, SP, Brasil |
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Abstract: | Summary Fifteen strains of yeast, which produced an extracellular amylolytic enzymes, were isolated from nature. One of them produced more than 100 times the enzyme activity in comparison with the 14 strains and the extremely hyperproducing strain of yeast was identified asCandida
sp. 347. Paper chromatograms of the amylolytic enzyme demonstrated activity of amyloglucosidase. The optimum pH for activity of the enzyme was 5.5–6.0 and optimum temperature was 60°C. |
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