Identification and characterization of a novel alternative splice variant of mouse GMx33alpha/GPP34 |
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Authors: | Nagano-Ito Michiyo Yoshikawa Sae Tamura Mika Tsurumaki Mihoko Ichikawa Shinichi |
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Institution: | Niigata University of Pharmacy and Applied Life Sciences (NUPALS), Laboratory for Animal Cell Engineering, 265-1 Higashijima Akiha-ku Niigata-shi Niigata 956-8603 Japan. |
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Abstract: | We have cloned and characterized a novel splice variant of mouse GMx33alpha/Golgi-associated protein of 34 kDa (GPP34), hereby designated GMx33alphaV/GPP34V. This splice variant skips the second and third exons, and the resulting frame shift generates a stop codon in the fourth exon. GMx33alphaV/GPP34V is comprised of 81 amino acid residues derived from the N-terminal end of the full length protein and corresponds to approximately one-third of the full length GMx33alpha/GPP34 sequence with a calculated molecular mass of 8900. In contrast to GMx33alpha/GPP34 mRNA which is expressed at similar levels in various tissues, GMx33alphaV/GPP34V mRNA was differentially expressed when examined by RT-PCR. Compared to other tissues, skeletal muscle showed relatively strong expression of GMx33alphaV/GPP34V mRNA. This splice variant cDNA was also detected in a human cell line. |
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Keywords: | Alternative splicing Golgi apparatus cDNA cloning Localization |
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