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| 猪流行性腹泻病毒嵌套式RT—PCR检测方法的建立 | |
| 引用本文： | 张素芳 贾赟 王敏秀 倪艳秀 何孔旺 陈溥言. 猪流行性腹泻病毒嵌套式RT—PCR检测方法的建立[J]. Virologica Sinica, 2004, 19(2): 174-176 |
| 作者姓名： | 张素芳 贾赟 王敏秀 倪艳秀 何孔旺 陈溥言 |
| 作者单位： | [1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室，江苏南京210095 [2]沈阳农业大学动物医学院，辽宁沈阳110161 [3]江苏省农业科学院农业部畜禽疫病诊断重点开放实验室，江苏南京210014 |
| 摘 要： | Two pairs of primers were designed according to the N gene sequence of PEDV-CV777 strain in Genebank (No. AF353511). PCR amplification product of outer-primers was 1328bp, and the inter-primers amplification product was 538bp, With the primers, a nested PCR assay was established to detect PEDV. This method was sensitive and specific and could be used in PEDV diagnosis and epidemiological investigation. |
| 关 键 词： | 猪流行性腹泻病毒 嵌套式RT-PCR检测方法 PED 诊断方法 分子生物学技术 |
Establishment of Nested RT-PCR Diagnostic Method for Porcine Epidemic Diarrhea Virus |
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| ZHANG Su-fang,,JIA Yun,WANG Min-xiu ,NI Yan-xiu,HE Kong-wang,CHEN Pu-yan. Establishment of Nested RT-PCR Diagnostic Method for Porcine Epidemic Diarrhea Virus[J]. 中国病毒学(英文版), 2004, 19(2): 174-176 | |
| Authors: | ZHANG Su-fang JIA Yun WANG Min-xiu NI Yan-xiu HE Kong-wang CHEN Pu-yan |
| Affiliation: | ZHANG Su-fang1,3,JIA Yun2,WANG Min-xiu 1,NI Yan-xiu3,HE Kong-wang3**,CHEN Pu-yan1 |
| Abstract: | Two pairs of primers were designed according to the N gene sequence of PEDV-CV777 strain in Genebank (No. AF353511). PCR amplification product of outer-primers was 1328bp, and the inter-primers amplification product was 538bp. With the primers, a nested PCR assay was established to detect PEDV. This method was sensitive and specific and could be used in PEDV diagnosis and epidemiological investigation. |
| Keywords: | Porcine epidemic diarrhea virus Nested-PCR Identification by restriction analysis |
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