二烯丙基二硫通过激活Caspase3和释放Cyt-c诱导Raji细胞凋亡 |
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引用本文: | 谭晖,吉晓霞,凌晖,罗招阳,何洁,易岚,周建国,苏琦.二烯丙基二硫通过激活Caspase3和释放Cyt-c诱导Raji细胞凋亡[J].生物物理学报,2009,25(1):22-28. |
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作者姓名: | 谭晖 吉晓霞 凌晖 罗招阳 何洁 易岚 周建国 苏琦 |
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作者单位: | 南华大学肿瘤研究所,湖南衡阳 421001 |
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基金项目: | 湖南省重点学科建设项目基金资助(2006-180),国家自然科学基金资助(30600285),湖南省自然科学基金资助(07JJ6155),湖南省教育厅科学研究项目(06C696) |
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摘 要: | 二烯丙基二硫(diallyl disulfide,DADS)作为天然植物大蒜中的提取物,能抑制多种肿瘤细胞生长,但其抑瘤的分子机制还不十分清楚。在该研究中,作者采用CCK-8(cell counting kit)技术检测发现,DADS能有效地抑制人淋巴瘤Raji细胞增殖,形态学观察、DNA琼脂糖凝胶电泳和流式细胞仪检测证实DADS呈时间和浓度依赖性诱导Raji细胞凋亡, DADS处理细胞24 h后, MCL1和Bcl-2蛋白表达下降,而Bax 和Bak蛋白表达水平无变化,Bid和Caspase3被激活,线粒体中Cyt-c释放增多,用Caspase 抑制剂Z-VAD-FMK能部分阻断DADS诱导人淋巴瘤Raji细胞凋亡, 提示DADS诱导的Raji细胞凋亡作用通过Bcl-2/MCL1-线粒体-caspase3通路介导。
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关 键 词: | DADS Raji细胞 凋亡 Caspase3 |
收稿时间: | 2008-12-18 |
Diallyl Disulfide-induced Apoptosis of Raji cells is Mediated by Caspase Activation and Cytochrome c Release |
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Institution: | Institute of Cancer Research, University of south china, Hengyang 421001, China |
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Abstract: | Diallyl disulfide (DADS) is a natural organosulfur compound derived from crushed garlic, it has been reported that the antiproliferative activity of DADS in several cancer cell lines. However, the molecular mechanism underlying the DADS-antitumor effect is not yet clearly understood. In the present study, the CCK-8 was used by assay the inhibition of DADS on the cells viability, the DADS-induced apoptosis in human Raji cells were investigated using both morphological analysis and DNA fragmentation technique. In this study, demonstrated that DADS-induced apoptosis in human Raji cells is a concentration- and time-dependent manner. This DADS-induced apoptosis in human Raji cells was also accompanied by the down-regulation of Bcl-2 and MCL-1 and activation of Bid, but no effect on the levels of Bax and Bak. Moreover, the DADS treatment resulted in increase in the release of mitochondrial cytochrome c (cyt c), and activation of caspase 3. Furthermore, treatment with caspase inhibitor (z-DEVD-fmk) was capable of preventing the DADS-induced caspase 3 activity and cell death. These results clearly demonstrate that the induction of apoptosis by DADS involves be mediated, at least in part, by the Bcl-2/MCL1-mitochondria-Caspase3 pathway in human Raji cells. |
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Keywords: | Diallyl disulfide Raji cells Apoptosis Caspase3 |
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