| 山茱萸不同栽培品种的 rDNA ITS 序列分析 |
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| 引用本文： | 陈随清,潘成学,卢小蕾,王利丽.山茱萸不同栽培品种的 rDNA ITS 序列分析[J].武汉植物学研究,2010,28(1):72-75. |
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| 作者姓名： | 陈随清 潘成学 卢小蕾 王利丽 |
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| 作者单位： | 1. 河南中医学院药学院,郑州,450008
2. 郑州大学,郑州,450052 |
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| 基金项目： | 科技部科技支撑计划项目 |
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| 摘 要： | 为测定山茱萸（Cornus officinalis Sieb.et.Zucc.）核糖体DNA的ITS序列,对山茱萸不同栽培品种进行了ITS序列分析。通过实验筛选出一对引物,进行PCR扩增,对扩增产物提取纯化,双脱氧链终止法DNA测序。然后,利用DNAssist Version 2.0软件加手工校正确定ITS1-5.8S-ITS2序列,并进行ITS序列分析。获得了山茱萸的ITS1-5.8S-ITS2完全序列,ITS1为253bp,5.8S为156bp,ITS2为273bp,总共682bp。7种果型的山茱萸其5.8S基因序列显示高度的一致性,圆柱形果型、长梨形果型、椭圆形果型和纺锤形果型的ITS区序列完全一致,短圆柱形果型在ITS1区3′端及ITS2区5′端各有1个变异位点;短梨形果型在ITS1区5′端有3个变异位点;长圆柱形果型在ITS1区有5个变异位点。结果表明,ITS序列在山茱萸种内比较保守,有的栽培品种之间有较小的差异,此研究为中药山茱萸分子鉴定提供了科学依据。
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| 关 键 词： | 山茱萸 栽培品种 rDNA ITS序列 |
rDNA ITS Sequence Analysis of Different Cultivar Variety of Cornus officinalis |
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| CHEN Sui-Qing,PAN Cheng-Xue,LU Xiao-Lei,WANG Li-Li.rDNA ITS Sequence Analysis of Different Cultivar Variety of Cornus officinalis[J].Journal of Wuhan Botanical Research,2010,28(1):72-75. |
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| Authors: | CHEN Sui-Qing PAN Cheng-Xue LU Xiao-Lei WANG Li-Li |
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| Institution: | CHEN Sui-Qing1,PAN Cheng-Xue2,LU Xiao-Lei1,WANG Li-Li1(1.Henan College of Traditional Chinese Medicine,Zhengzhou 450008,China,2.Zhengzhou University,Zhengzhou 450052,China) |
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| Abstract: | To study rDNA ITS sequence of Cornus officinalis Sieb.et.Zucc.,and analyzed the rDNA ITS sequence of its different cultivated varieties.A pair of primers was selected by experiment.The ITS gene fragment was amplified using this pair of primers,and PCR products were purified with DNA gel extraction kit(AxyPrepTM)and sequenced by the method of Sanger dideoxy.Then the ITS1-5.8S-ITS2 sequences were analyzed by the software of DNAssist Version 2.0.The ITS1-5.8S-ITS2 sequences was found whole ITS sequence consist... |
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| Keywords: | rDNA Comus officinalis Cultivated varieties rDNA ITS sequence |
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